Scientist I

San Diego, CA, US • Posted 1 day ago • Updated 21 hours ago
Contract Independent
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  • Scientist I

Summary

Greetings from IT Engagements
IT Engagements is a global staff augmentation firm providing a wide-range of talent on-demand and total workforce solutions. We have an immediate opening for the below position with one of our premium clients.
Job Summary
Job Title: Scientist I (Contractor)
Dept: Genetic Medicine Technology (gRED)
Work Location: San Diego, CA
Working Model: Fully Onsite (Monday through Friday)
Interview Model: Two rounds (Virtual - 30 minutes each)
Description
Position Summary:
The Scientist I will be a vital contributor to our non-viral cell and gene therapy delivery platform, primarily focused on R&D for innovative nucleic acid payload technologies. This role involves evaluating novel mRNA and DNA designs using a range of molecular biology techniques, including plasmid cloning, in vitro validation, and the production of in vitro-transcribed mRNA. To verify successful expression, the scientist will utilize assays such as Western blot, qRT-PCR, and luciferase reporter assays.
Responsibilities:
  • Design and clone expression vectors and IVT donor plasmids to support research initiatives.
  • Execute DNA and mRNA transfections in mouse and human cell lines.
  • Assess nucleic acid expression through molecular methods like qPCR, reporter assays and Western blotting.
  • Maintenance of detailed electronic laboratory notebooks (ELN) and preparation of summary slide
Requirements, Knowledge, Skills, and Abilities:
  • Education/Experience: Bachelor's degree with 5+ years or a Master's degree with 2+ years of experience in molecular biology, pharmaceutical sciences, or a related field.
  • Molecular Biology: Required proficiency in advanced cloning (Gibson assembly, Golden Gate, site-directed mutagenesis) and IVT.
  • Functional Assays: Hands-on expertise in measuring transgene delivery success via reporter assays and qRT-PCR.
  • Cell Culture: Preferred experience in genetic manipulation of cell lines and primary cells (e.g., T cells, monocytes, hepatocytes).
  • Software/Tools: Desired proficiency in Snapgene, Prism, and MS Office.
  • Communication: Exceptional oral and written skills with a meticulous attention to detail.
Thanks
Divya
Employers have access to artificial intelligence language tools (“AI”) that help generate and enhance job descriptions and AI may have been used to create this description. The position description has been reviewed for accuracy and Dice believes it to correctly reflect the job opportunity.
  • Dice Id: 91119149
  • Position Id: 2026-2220
  • Posted 1 day ago
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